Determination of Possible Adulteration and Quality Assessment in Commercial Honey

Abstract

This study aims to predict several quality traits in commercial honey samples simultaneously and to reveal possible honey adulteration using a field-deployable portable infrared spectrometer without any sample preparation. A total of one hundred and forty-seven commercial honey samples were purchased from local and online markets in Turkey and the United States of America (USA), and their soluble solids (°Brix), pH, free acidity, moisture, water activity (aw), glucose, fructose, sucrose, and hydroxymethyl furfural (HMF) contents were determined using reference methods. The HMF (n = 11 samples) and sucrose (n = 21) concentrations were higher than the regulatory limits in some tested samples. The exceeding HMF content may imply temperature abuse during storage and prolonged storing. On the other hand, high sucrose content may indicate possible adulteration with commercial sweeteners. Therefore, soft independent modeling of class analogies (SIMCA) analysis was conducted to reveal this potential sweetener adulteration in the samples, and the SIMCA model was able to identify all the flagged samples. The suggested FT-IR technique may be helpful in regulatory bodies in determining honey authenticity issues as well as assessing the quality characteristics of honey samples in a shorter period and at a lower cost.