Over the last decade, science has greatly improved in the area of protein sizing and characterization. Efficient high-throughput methods are now available to substitute for the traditional labor-intensive SDSPAGE methods, which alternatively take days to analyze a very limited number of samples. Currently, PerkinElmer® (Caliper) has designed an automated chip-based fluorescence detection method capable of analyzing proteins in minutes with sensitivity similar to standard SDS-PAGE. Here, we describe the use and implementation of this technology to characterize and screen a large number of formulations of target glycoproteins in the 14-200 kDa molecular weight range. © Springer Science+Business Media New York 2013.
CITATION STYLE
Chen, G., Ha, S., & Rustandi, R. R. (2013). Characterization of glycoprotein biopharmaceutical products by caliper LC90 CE-SDS gel technology. Methods in Molecular Biology, 988, 199–209. https://doi.org/10.1007/978-1-62703-327-5_12
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