Methodical basis of analysis for various genesis of humic acids

Abstract

Our proposed methodology includes organic elemental and thermal analysis and IR and 13C NMR spectroscopy. In this case, the results obtained are the following set of quantitative parameters: mass fraction (%) of C, H, N and O in ash-free sample; the atomic ratio of H/C, C/N and O/C; and the relative content (%) of carboxyl, aromatic, aliphatic and polysaccharide fragments in the macromolecule. Outlines of the IR and NMR spectra and thermograms are used to assess the qualitative differences between humic acids.