Identification of microorganisms using digital holographic microscopy

Abstract

The recognition of microorganisms is important in the diagnosing of the micro-biology. In the medical field the cell disorders can affect body tissues indirectly. In this work we use digital holographic microscopy (DHM) that can achieve high contrast images. This method permits us to obtain phase maps by means of digital reconstruction of a wavefront. Phase differences between two states allows us to realize a quantitative analysis of samples. An off-axis layout is used to determine the morphology of different types of microorganisms. Also, we can directly examine aggregations by corresponding phase maps. The images provide information about the presence or absence of microorganisms that can be used as indicators of the physiological state of the body. By the presented results, we show that DHM may give some new perspectives within biomedical applications.