Full-length cDNAs are essential for the correct annotation of transcriptional units and gene products from genomic sequence data and for functional analysis of the genes. Full-length cDNA libraries are very important resources for isolation of the full-length cDNAs. The biotinylated cap trapper method using the trehalose-thermostabilized reverse transcriptase has been developed and has become an efficient method for construction of high-content full-length cDNA libraries. We have constructed full-length cDNA libraries from various plants and animals using this method. The protocol of the method is described in this chapter. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Seki, M., Kamiya, A., Carninci, P., Hayashizaki, Y., & Shinozaki, K. (2009). Generation of full-length cDNA libraries: Focus on plants. Methods in Molecular Biology, 533, 49–68. https://doi.org/10.1007/978-1-60327-136-3_4
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