The plant perception of pathogen-associated molecular patterns triggers a plethora of cellular immune responses. One of these responses is a rapid and transient burst of reactive oxygen species (ROS) mediated by plasma membrane-localized NADPH oxidases. The ROS burst requires a functional receptor complex and the contribution of several additional regulatory components. In laboratory conditions, the ROS burst can be detected a few minutes after the treatment with an immunogenic microbial elicitor. For these reasons, the elicitor-triggered ROS burst has been often exploited as readout to probe the contribution of plant components to early immune responses. Here, we describe a detailed protocol for the measurement of elicitor-triggered ROS burst in a simple, fast, and easy manner.
CITATION STYLE
Sang, Y., & Macho, A. P. (2017). Analysis of PAMP-triggered ROS burst in plant immunity. In Methods in Molecular Biology (Vol. 1578, pp. 143–153). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6859-6_11
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