Mass spectrometric analysis of epimeric 3α/3β functionalized steroids

Abstract

Tandem mass spectrometry (MS/MS) using an atmospheric pressure chemical ionization (APCI) source in positive mode (+) reveals different intensity ratios of two fragment peaks of the epimeric cholestane derivatives synthesized from 3α- or 3β-cholestanol. The investigation of the fragmentation pattern obtained from their full scan and/or collision induced dissociation (CID) spectra in the case of the cholestane skeleton together with the respective electronic ionization (EI) spectra supports a distinction of the epimers. ©ARKAT USA, Inc.