The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.
CITATION STYLE
Murungi, L. M., Kimathi, R. K., Tuju, J., Kamuyu, G., & Osier, F. H. A. (2019). Serological profiling for malaria surveillance using a standard ELISA protocol. In Methods in Molecular Biology (Vol. 2013, pp. 83–90). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9550-9_6
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