Alteration of Citrate Metabolism in Cluster Roots of White Lupin

50Citations
Citations of this article
32Readers
Mendeley users who have this article in their library.

Abstract

Organic acid excretion plays a key role in the superior P i-acquisition of barely soluble inorganic P sources from soils. Seedlings of white lupin (Lupinus albus L.) grown for 37 d in -P nutrient solution showed typical -P symptoms, such as low P content, increased root/ shoot ratio and the development of cluster roots which released large amounts of citrate. Citrate concentration in the cluster roots was 21.5 μmol (g FW)-1, which corresponded to a 4.3- and 2.6-fold increase of +P and -P root apexes, respectively. Cluster roots possessed higher phosphoenolpyruvate carboxylase and phosphoenolpyruvate phosphatase activity than those in +P root apexes, which could result in increasing the supply of substrate for citrate synthase. On the other hand, the cytosolic pathway which converts citrate to 2-oxoglutarate consists of aconitase and NADP-specific isocitrate dehydrogenase activity that was lower in the cluster roots than in +P root apexes, and may contribute to citrate accumulation. Thus, metabolic balance with these alterations would play an important role in increasing citrate concentration in the cluster roots. The molecular characterization of NADP-specific isocitrate dehydrogenase indicated that the cytosolic isoenzyme functions as a hetero-dimer, and that the activity would be regulated by the transcript levels for both isoforms.

References Powered by Scopus

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

232748Citations
N/AReaders
Get full text

[1] Citrate synthase. [EC 4.1.3.7. Citrate oxaloacetate-lyase (CoA-acetylating)]

2096Citations
N/AReaders
Get full text

Function and mechanism of organic anion exudation from plant roots

1217Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Sucrose transport in the phloem: Integrating root responses to phosphorus starvation

406Citations
N/AReaders
Get full text

Cluster roots: A curiosity in context

344Citations
N/AReaders
Get full text

Enhancing plant phosphorus use efficiency for sustainable cropping

260Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Kihara, T., Wada, T., Suzuki, Y., Hara, T., & Koyama, H. (2003). Alteration of Citrate Metabolism in Cluster Roots of White Lupin. Plant and Cell Physiology, 44(9), 901–908. https://doi.org/10.1093/pcp/pcg115

Readers over time

‘11‘12‘13‘14‘15‘16‘17‘18‘19‘20‘21‘22‘23‘2402468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 12

52%

Researcher 7

30%

Professor / Associate Prof. 3

13%

Lecturer / Post doc 1

4%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 20

83%

Biochemistry, Genetics and Molecular Bi... 2

8%

Medicine and Dentistry 1

4%

Earth and Planetary Sciences 1

4%

Save time finding and organizing research with Mendeley

Sign up for free
0