Development of a Microtiter Plate Enzyme-linked Immunosorbent Assay for 17α, 20β-21-trihydroxy-4-pregnen-3-one, a Teleost Gonadal Steroid

Abstract

A simple solid-phase microtiter plate enzyme-linked immunosorbent assay (ELISA) for 17α, 20β, 21-trihydroxy-4-pregnen-3-one (20β-S), a teleost maturation inducing steroid, was developed using 20β-S-3-CMO-horseradish peroxidase as the label, and a homologous antiserum raised in rabbits. Second antibody coated to the plate was used to capture the antiserum with labeled or unlabeled 20β-S. Within three hours, a steep standard curve covering 1.5-192 pg/well was obtained. The sensitivity and precision of this ELISA was almost the same as those of ordinary steroid radioimmunoassays. This non-isotopic assay system also allows prompt measurement of 20β-S in catfish plasma with good specificity. © 1995, The Japanese Society of Fisheries Science. All rights reserved.