Bioindication for the search of microorganisms-destructors

Abstract

The work is devoted to the substantiation of soil sampling points for the selection of promising strains-destructors of organic pollutants using bioindication to reduce the volume of routine microbiological studies, by excluding samples taken in environmentally friendly locations and focusing on samples from environmentally “dirty” points. The paper presents the results of research carried out in 2018–2019. In the course of work, 11 points were studied in obviously environmentally friendly and environmentally unfriendly locations on the territory of Krasnodarskiy krai (Anapa, Novorossiysk and Gelendzhik district) and, respectively, 14 points in obviously environmentally friendly and environmentally unfriendly locations in the North-West region (Saint-Petersburg, suburbs and Leningrad oblast) of Russia. Pinus brutia var. Pityusa (STEVEN) SILBA, 1985 for Krasnodarskiy krai; Pinus sylvestris L., 1753 and Quercus robur L., 1753 for the North-West region of Russia were used as indicator plants. As an indicator – a joint test criterion for zero asymmetry and excess of distribution of the maximum length of needles of Pinus brutia var. Pityusa (STEVEN) SILBA, 1985; Pinus sylvestris L., 1753; weight of acorns of Quercus robur L., 1753. The proposed method showed the simplicity of primary data collection and high sensitivity. Low requirements for the accuracy of primary measurements (as opposed to fluctuating asymmetry) allow future automatization of the process using a smartphone camera and the use of the method not only to search for promising strains-destructors, but also to develop a mobile application for assessing the local environmental situation for a wide range of users.