Metal affinity precipitation has been successfully developed as a simple purification process for the proteins that have affinity for the metal ions. The copolymers of vinylimidazole with N-isopropylacrylamide are easily synthesized by radical polymerization. When loaded with Cu(II) and Ni(II) ions, these copolymers are capable of selectively precipitating proteins with natural metal-binding groups or histidine-tagged recombinant proteins. © Humana Press.
CITATION STYLE
Kumar, A., Galaev, I. Y., & Mattiasson, B. (2007). Affinity precipitation of proteins using metal chelates. Methods in Molecular Biology, 421, 37–52. https://doi.org/10.1007/978-1-59745-582-4_3
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