Abstract
The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.
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CITATION STYLE
Sivakumaran, K., Chen, M. H., Roossinck, M. J., & Kao, C. C. (2002). Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A. Molecular Plant Pathology, 3(1), 43–52. https://doi.org/10.1046/j.1464-6722.2001.00089.x
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