The binding of small molecules to non-canonical nucleic acid structures has been a major focus of rational drug design. Among the non-canonical nucleic acid structures, targeting poly(A) using small molecules has attracted a special interest due to the cellular functions of poly(A) tails. Here, the methods for determining the binding of a small molecule to poly(A) using UV-visible(UV–Vis) and Circular Dichroism (CD) Spectroscopy are described. Experiments used in determining the melting temperature, binding stoichiometry and dissociation constant of poly(A)-small molecule systems are depicted.
CITATION STYLE
Persil Çetinkol, Ö. (2014). Small molecule recognition of poly(A). Methods in Molecular Biology, 1125, 81–108. https://doi.org/10.1007/978-1-62703-971-0_8
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