GPCR microspot assays on solid substrates.

Abstract

Multiplexing and miniaturization make microarrays an attractive tool for biomolecular interaction analysis. Adequate shelf life, mechanical stability through multiple assay steps, and amenability to a microplate format for screening are core requirements for the practical large-scale implementation of microarray technology. G protein-coupled receptor (GPCR) microarrays require the co-immobilization of the receptors and their associated lipid membranes. The vulnerability of solid-supported membranes to desorption and the unique surface requirements for GPCR function provide formidable challenges for the fabrication of GPCR microarrays. The chapter describes air-stable GPCR microarrays and their utility for selectivity profiling of GPCR drugs with high fidelity.