Building a two-photon microscope is easy because most of the work is done by the laser itself. All the microscope needs to do is to focus the laser light to a point, move it across the preparation, and measure the fluorescence photons emitted. These jobs are done by an objective, a scan engine, and a detector, respectively. That’s all there is to it.
CITATION STYLE
Smith, S. L. V. (2019). Building a two-photon microscope is easy. In Neuromethods (Vol. 148, pp. 1–16). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9702-2_1
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