Microarrays for high-throughput gene expression analysis of barley

Abstract

Microarrays remain an extremely robust and cost-efficient technology for transcriptome analysis. A custom Agilent microarray has been designed from the predicted gene transcripts of the barley genome reference. Here, we describe total RNA extraction, fluorescent labeling, hybridization, and image analysis of barley arrays. Quality control measures are detailed, along with recommendations for downstream data analysis.