Two New Potent Inhibitors of Xanthine Oxidase from Leaves of Perilla Frutescens Britton Var: Acuta Kudo

Abstract

A known and a new caffeic ester (1 and 2), new inhibitors of xanthine oxidase (XO)f were isolated from leaves of Perilla frutescens var. acuta and their structures have been established as (Z,E)-2-(3,4-dihydroxyphenyl)ethenyl ester (1) and (Z,E)-2-(3,5-dihydroxyphenyl)ethenyl ester (2) of 3 - (3,4-dihydroxypheny 1)-2-propenoic acid, respectively, based on detailed spectral studies, including 2D COSY, long range COSY, difference NOE, etc. Both caffeic esters strongly inhibited XO in vitro and especially, the inhibition by 1 was as potent as that by allopurinol. The inhibition mode of 1 was also shown to be non-competitive. © 1990, The Pharmaceutical Society of Japan. All rights reserved.