In cells, the poly(A) tail stimulates translation from messenger RNAs bearing a cap structure or viral IRES elements. This 3' end-mediated stimulation of translation is not reflected in commonly used commercial cell-free translation systems prepared from rabbit reticulocytes or wheat germ. We describe a simple procedure to generate poly(A) tail-responsive translation extracts from HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines.
CITATION STYLE
Schoenberg, D. R. (2004). mRNA Processing and Metabolism. mRNA Processing and Metabolism. Humana Press. https://doi.org/10.1385/1592597505
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