Use of next generation sequencing (NGS) technologies for the genome-wide detection of transposition

Abstract

Plant transposable elements are ubiquitous in eukaryotes. Their propensity to densely populate the genomes of many plants and animal species has put them in the focus of both structural and functional genomics. Although a number of bioinformatic software have been recently developed for the annotation of TEs in sequenced genomes, there are very few computational tools strictly dedicated to the identification of active TEs using genome-wide approaches. In this paper, we describe SearchTESV, a pipeline that we have developed to detect Transposable Elements-associated structural variants (TEASVs) using Next Generation Sequencing (NGS) technologies. © Springer Science+Business Media, New York 2013.