Early inflammatory reactions in atherosclerosis are induced by proline-rich tyrosine kinase/reactive oxygen species-mediated release of tumor necrosis factor-α and subsequent activation of the p21Cip1/Ets-1/p300 system

Abstract

Objective- Reactive oxygen species (ROS) are involved in the initial process of atherosclerosis, whereas it remains to be determined how atherogenic stimulus causes ROS-mediated proinflammatory reactions. Here, we focused on proline-rich tyrosine kinase (PYK2)-mediated ROS generation and examined how atherogenic stimulus causes early proinflammatory reactions. Methods and Results- PYK2-deficient (knockout [KO]) (PYK2-KO) mice were crossbred with apolipoprotein E (apoE)-deficient (PYK2-KO/apoE-KO) mice. PYK2-KO/apoE-KO mice and endothelial cells (EC) were used for the study. Aortic atherogenic lesions in PYK2-KO/apoE-KO mice were markedly decreased (55% versus apoE-KO) after 8 weeks of a Western diet. Aortic PYK2 was activated as early as 7 days after the Western diet, when inflammatory cells were not yet activated. Addition of the proatherogenic oxidized phospholipid lysophosphatidylcholine caused activation of endothelial PYK2. Lysophosphatidylcholine-activated PYK2 induced NADPH oxidase-mediated ROS generation and ROS-mediated synthesis of tumor necrosis factor-α (TNFα), vascular cell adhesion molecule-1 (VCAM-1), monocyte chemotactic protein-1 (MCP-1), and p21/Ets-1. Neutralizing anti-TNFα antibody or knockdown of p21/Ets-1 system blocked the induction of VCAM-1 and MCP-1. PYK2 deficiency abolished these ROS-mediated proinflammatory reactions. Further analysis revealed that PYK2/ROS-mediated p21/Ets-1 activation upregulated the transcription of the MCP-1 gene in collaboration with p300 transcription coactivator. Conclusion- PYK2 is a key tyrosine kinase activated by high cholesterol exposure, which causes ROS-mediated TNFα release and induces TNFα-dependent expression of proinflammatory molecules via the p21/Ets-1/p300 transcription system. Copyright © 2011 American Heart Association. All rights reserved.