An electrochemical molecularly imprinted polymer sensor for rapid β-lactoglobulin detection

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Abstract

Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor’s selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10−9 and 10−4 mg/mL, and a detection limit of 10−9 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample.

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Wang, B., Hong, J., Liu, C., Zhu, L., & Jiang, L. (2021). An electrochemical molecularly imprinted polymer sensor for rapid β-lactoglobulin detection. Sensors, 21(24). https://doi.org/10.3390/s21248240

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