Effects of haemoperfusion on selected indices of blood biochemistry in sheep

Abstract

The aim of this work was to evaluate the clinoptilolite as a cartridge for haemoperfusion columns. Changes in haematological indices and blood plasma macro-elements were evaluated during haemoperfusion in sheep with the use of zeolitic and commercial haemoperfusion columns. Haemoperfusions were done in two groups of sheep (n=6) for 2 hours. In the first group, commercial haemoperfusion columns HEMASORB 400 C filled with active charcoal were used. In the second group, the columns used were filled with sodium form of natural zeolite-clinoptilolite partially saturated with potassium chloride. During 2 hours of haemoperfusion significant decrease in numbers of leukocytes and thrombocytes (P<0.01) were found in both types of columns. There were no significant differences between the groups, except for the number of thrombocytes and plasma sodium concentration in the blood leaving the zeolitic columns with significantly lower values after 30 min (134.3±28.9 vs. 251.0±55.4 G.1-1, P<0.01; and 144.17±9.13 vs. 157.17±7.36 rnmol.l-1, P<0.05; respectively) compared with commercial HEMASORB columns. However, final evaluation of biocompatibility of zeolitic haemoperfusion columns will require more detailed research.