We present a novel concept to process human blood on a spinning polymer disk for the determination of the hematocrit level by simple visual inspection. The microfluidic disk which is spun by a macroscopic drive unit features an upstream metering structure and a downstream blind channel where the centrifugally enforced sedimentation of the blood is performed. The bubble-free priming of the blind channel is governed by centrifugally assisted capillary filling along the sloped hydrophilic side-wall and the lid as well as the special shape of the dead end of the two-layer channel. The hematocrit is indicated at the sharp phase boundary between the plasma and the segregated cellular pellet on a disk-imprinted calibrated scale. This way, we conduct the hematocrit determination of human blood within 5 min at a high degree of linearity (R2 = 0.999) and at a high accuracy (CV = 4.7%) spanning over the physiological to pathological working range. As all processing steps including the priming, the metering to a defined volume as well as the centrifugation are executed automatically during rotation, the concept is successfully demonstrated in a conventional PC-CDROM drive while delivering the same performance (R2 = 0.999, CV = 4.3%). © Springer Science+Business Media, LLC 2007.
CITATION STYLE
Riegger, L., Grumann, M., Steigert, J., Lutz, S., Steinert, C. P., Mueller, C., … Ducrée, J. (2007). Single-step centrifugal hematocrit determination on a 10-$ processing device. Biomedical Microdevices, 9(6), 795–799. https://doi.org/10.1007/s10544-007-9091-1
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