Preparation of modified RNA sequences for biological research

Abstract

Our group is working on the development of reliable access to biologically relevant, long, and modified RNA sequences. Here, we briefly present the chemical synthesis of such sequences with 2′-O-triisopropylsilyloxymethyl (= 2′-O-tom) protected ribonucleoside phosphoramidites, their template-directed enzymatic ligation, and some examples of modified nucleotides, designed to promote structural and biological studies. © Schweizerische Chemische Gesellschaft.