Adventitious Shoot Regeneration and Plant Production from Explants of Apios americana

Abstract

Shoots were regenerated from callus of Apios americana Medikus (apios, groundnut) using internodal explants from in vitro-germinated seedlings and from sprouted tubers on a modified Murashige and Skoog (MS) basal medium. Shoot regeneration was observed over a range of 2iP and IBA combinations. GA 3 increased the number of shoots regenerated per epicotyl explant. The most efficient regeneration (≈90%) was with internodal epicotyl explants on 100 μ m 2iP, 0.5 μ m IBA, and 1.5 μ m GA 3 . Regenerated shoots were rooted on liquid and solid MS medium with 0.5 μ m IBA; however, rooting was more successful on the liquid medium. About 60% of rooted plants were successfully established in pots. Chemical names used: N- (3-methyl2-butenyl)-1 H- purin-6-amine (2iP), 1 H- indole-3-butanoic acid (IBA), gibberellic acid (G A 3 ).