Phytochemical and toxicity test with the BSLT method on Kemuning Raya leaf extract (Muraya paniculata L.)

Abstract

Phytochemical and toxicity tests have been carried out using the BSLT method on kemuning leaves. The kemuning leaves are picked in the morning before sunrise then washed, then dried for 5 days, then blended and sieved to obtain kemuning leaves powder. Furthermore, the phytochemical screening test obtained shows the presence of alkaloids that form red deposits (precipitate) with dragendroff reagent, yellowish white deposits (precipitate) with mayer reagents, and the presence of flavonoids is indicated by the formation of black colloids. The most flavonoid yields were obtained (the biggest secondary metabolites components in the extract are flavonoids), while the presence of saponins was indicated by the presence of foam that lasted ±10 minutes. Tannins form dark blue or greenish black rings. While steroids and terpenoids marked with brown or violet rings are not formed and not present in kemuning leaf extract. The results of the toxicity test with BSLT showed moderate toxicity to shrimp larvae.