Abstract
In this chapter we describe the workflow used in our laboratory to analyze rice leaf samples using label-free shotgun proteomics based on SDS-PAGE fractionation of proteins. Rice proteomics has benefitted substantially from successful execution of shotgun proteomics techniques. We describe steps on how to proceed starting from rice protein extraction, SDS-PAGE, in-gel protein digestion with trypsin, nanoLC-MS/MS, and database searching using the GPM. Data from these experiments can be used for spectral counting, where simultaneous quantitation of several thousand proteins can be obtained. © 2014 Springer Science+Business Media, LLC.
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Neilson, K. A., George, I. S., Emery, S. J., Muralidharan, S., Mirzaei, M., & Haynes, P. A. (2014). Analysis of rice proteins using SDS-PAGE Shotgun Proteomics. Methods in Molecular Biology, 1072, 289–302. https://doi.org/10.1007/978-1-62703-631-3_21
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