Optical manipulation is a powerful way to control neural activity in vitro and in vivo with millisecond precision. Patterning of light provides the remarkable ability to simultaneously target spatially segregated neurons from a population. Commercially available projectors provide one of the simplest and most economical ways of achieving spatial light modulation at millisecond timescales. Here, we describe the protocol for constructing a projector-based spatio-temporal light patterning system integrated with a microscope on a typical electrophysiology rig. The set-up is well suited for applications requiring rapid, distinct, and combinatorial inputs, akin to brain activity. This equipment involved is fairly economical (
CITATION STYLE
Bhatia, A., Moza, S., & Bhalla, U. S. (2021). Patterned optogenetic stimulation using a DMD projector. In Methods in Molecular Biology (Vol. 2191, pp. 173–188). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0830-2_11
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