Synthesis of thermal phase separating reactive polymers and their applications in immobilized enzymes

133Citations
Citations of this article
17Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

This paper describes the synthesis of active ester copolymers from N-isopropylacrylamide (NIP) with N-acryloxysuccinimide (NAS), aminolysis of active esters, and determination of their average molecular weights. As a model, thermolysin was attached to the polymer to form soluble-insoluble immobilized enzymes capable of being centrifuged above their Tc, because poly(N-isopropylacylamide) (poly(NIP)) has a lower critical solution temperature (Tc) of about 31舑33ŶC. The effects of immobilization of enzymes have been studied including the amount of active ester, time, pH, temperature, and ratio of polymer to enzyme. The tests of thermal stability and repeated precipitation and separation showed that the use of Tc as the technique of separation is possible. © 1993, The Society of Polymer Science, Japan. All rights reserved.

Cite

CITATION STYLE

APA

Liu, F., Tao, G., & Zhuo, R. (1993). Synthesis of thermal phase separating reactive polymers and their applications in immobilized enzymes. Polymer Journal, 25(6), 561–567. https://doi.org/10.1295/polymj.25.561

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free