High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and Proteins Extraction with FastPrep® Systems

Abstract

The well recognized “bottleneck” in sample preparation of the difficult samples is efficient lysis, homogenization, and grinding of the sample. Herein we describe the principles of operations and applications of the FastPrep® family of systems and kits for lysing, homogenization, and grinding, utilizing the modified bead beating technique based on the combination of simultaneous cascade impaction, mechanical shearing and hydrodynamic/vortex shearing. The chapter provides an overview of the systems, lysing matrices and application examples relevant to efficient lysis, grinding, and homogenization for downstream molecular biology, proteomics, or general biochemistry sample prep workflow for animals, plants, microbes, and environmental samples which are difficult to lyse