To identify cytosolic proteins that bind to cyclic di-AMP, a biotinylated analog of the nucleotide is used for protein pull-down experiments. In this approach, biotinylated c-di-AMP is coupled to Streptactin-covered beads. After protein separation using standard SDS-PAGE, the protein(s) of interest are identified by mass spectrometric analyses.
CITATION STYLE
Kampf, J., Gundlach, J., Herzberg, C., Treffon, K., & Stülke, J. (2017). Identification of c-di-AMP-binding proteins using magnetic beads. In Methods in Molecular Biology (Vol. 1657, pp. 347–359). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7240-1_27
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