Hydrogels based on peptide self-assembly form an important class of biomaterials that find application in tissue engineering and drug delivery. It is essential to prepare peptides with high purity to achieve batch-to-batch consistency affording hydrogels with reproducible properties. Automated solid-phase peptide synthesis coupled with optimized Fmoc (9-fluorenylmethoxy- carbonyl) chemistry to obtain peptides in high yield and purity is discussed. Details of isolating a desired peptide from crude synthetic mixtures and assessment of the peptide's final purity by high-performance liquid chromatography and mass spectrometry are provided. Beyond the practical importance of synthesis and primary characterization, techniques used to investigate the properties of hydrogels are briefly discussed. © 2008 Humana Press.
CITATION STYLE
Nagarkar, R. P., & Schneider, J. P. (2008). Synthesis and primary characterization of self-assembled peptide-based hydrogels. Methods in Molecular Biology, 474, 61–77. https://doi.org/10.1007/978-1-59745-480-3_5
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