Fluorescence in situ hybridization (FISH) method enables in situ genetic analysis of both metaphase and interphase cells from different types of material, including cell lines, cell smears, and fresh and paraffin-embedded tissue. Despite the growing number of commercially available FISH probes, still for large number of gene loci or chromosomal regions commercial probes are not available. Here we describe a simple method for generating FISH probes using bacterial artificial chromosomes (BAC). Due to genome-wide coverage of BAC clones, there are almost unlimited possibilities for the analysis of any genomic regions using BAC FISH probes.
CITATION STYLE
Stankiewicz, E., Guo, T., Mao, X., & Lu, Y. J. (2019). Fluorescence in situ hybridization and rehybridization using bacterial artificial chromosome probes. In Methods in Molecular Biology (Vol. 2054, pp. 243–261). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9769-5_16
Mendeley helps you to discover research relevant for your work.