Analytical method by micro-elisa for small amount of patient sample

Abstract

Previously, we developed a new functional device (μELISA) by applying microfluidics and thermal-lens microscope. It has been clarified from our research that μELISA exhibits excellent performance for measuring human serum. However, when the analysis is performed for a very small amount of various patient samples in microliter order, differences in the composition or viscosity of each sample may effect the measurement values. In this research, the measurement conditions were determined for real patient serum by utilizing CRP. As a result, it has been confirmed that there is some effect that originates from some difference of each patient’s serum. To obtain reliable measured values, it is necessary to dilute each sample by a buffer.