A simple analytical workflow is described for gas chromatographic-mass spectrometry (GC-MS)-based metabolomic profiling of protic metabolites, particularly amino-carboxylic species in biological matrices. The sample preparation is carried out directly in aqueous samples and uses simultaneous in situ heptafluorobutyl chloroformate (HFBCF) derivatization and dispersive liquid-liquid microextraction (DLLME), followed by GC-MS analysis in single-ion monitoring (SIM) mode. The protocol involves ten simple pipetting steps and provides quantitative analysis of 132 metabolites by using two internal standards. A comment on each analytical step and explaining notes are provided with particular attention to the GC-MS analysis of 112 physiological metabolites in human urine.
CITATION STYLE
Hušek, P., Švagera, Z., Hanzlíková, D., Karlínová, I., Řimnáčová, L., Zahradníčková, H., & Šimek, P. (2018). GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol. In Methods in Molecular Biology (Vol. 1738, pp. 159–181). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7643-0_11
Mendeley helps you to discover research relevant for your work.