Optimizing Suitable Conditions for the Removal of Ammonium Nitrogen by a Microbe Isolated from Chicken Manure

Abstract

Strain C was isolated from chicken manure, and its phenotypic characteristics were gram-stain negative, yellow-pigmented, aerobic bacterium, heterotrophic, non-motile, chemoorganotrophic, non-gliding as well as non-spore-forming. A 16S rRNA gene sequence analysis showed that strain C occupied a distinct lineage within the family of the genus Chryseobacterium, and it shared highest sequence similarity with Chryseobacterium solincola strain 1YB-R12 (80%). The new isolate has been studied for removing ammonium-nitrogen (NH4-N) and the optimization of suitable conditions. The strain C was able to degrade over 42.8% of NH4-N during its active growth cycle. Experimental study of the effect of temperature and pH on NH4-N removal showed that the temperature and pH optima for NH4-N removal were 30-35°C and 4-8, respectively. The results indicated that strain C shows a potential application for wastewater treatment.