The study of regulatory T cells (Treg) requires methods for both in vivo and in vitro analyses, both of which have different limitations, but which complement each other to give a more complete picture of physiological function than either method alone. Our analyses have focused on Treg-mediated suppression of CD8+ T cells, and in particular Tregs induced by viral infection. One of the unique characteristics of virus-induced Tregs is that they can suppress CD8+ T cell function in vitro without the requirement for additional stimulation. This ability correlates with their suppressive capacity and activated status in vivo. Interestingly, while virus-induced Tregs suppress CD8+ T cell function in vitro and in vivo, they do not suppress proliferation unless they are further activated in vitro.
CITATION STYLE
Hasenkrug, K. J., & Myers, L. M. (2011). In Vitro and In Vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells. In Methods in Molecular Biology (Vol. 707, pp. 45–54). Humana Press Inc. https://doi.org/10.1007/978-1-61737-979-6_4
Mendeley helps you to discover research relevant for your work.