Separation of the neuroproteome by ion exchange chromatography

Abstract

Ion exchange chromatography is a fractionation technique applicable to the separation of brain-derived proteins based on charge. Proteome complexity overwhelms analytical approaches, which is mitigated by fractioning samples into simpler solutions. In this chapter we will cover the design, optimization, and execution of an ion exchange experiment for the separation of a brain lysate. Furthermore, helpful tips and pitfall will be revealed to aid with potential problems that may arise. The discussed proteomic methodology is applicable to multidimensional separations ahead of bottom-up or top-down proteomic strategies for characterizing the neuroproteome. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.