Molecular recognition of IL-8, IL-10, IL-12, and IL-15 in biological fluids using phthalocyanine-based stochastic sensors

Abstract

Two stochastic sensors based on the modification of graphite paste with the complexes formed by phthalocyanine (PhCN) with Ni and Cu were designed and used for molecular recognition of IL-8, IL-10, IL-12, and IL-15. The four interleukins were recognized according to their signatures—called toff (qualitative parameter) from the diagrams obtained after measurements. The limit of determination for IL-8 was 1 × 10−4μg mL−1 when both stochastic sensors were used; for IL-10, the determination limit was 4.5 × 10−4μg mL−1 for the Ni complex-based sensor, and 4.5 × 10−7μg mL−1 for the Cu complex-based sensor, respectively; for IL-12, the determination limit was 5 × 10−4μg mL−1 for the Ni complex-based sensor, and 5 × 10−7μg mL−1 for the Cu complex-based sensor, respectively; while for IL-15, the determination limit was 5 × 10−5μg/mL for the Ni complex-based sensor, and 5 × 10−5μg/mL for the Cu complex-based sensor, respectively. The stochastic method used was validated using the following biological fluids: nasal lavage, saliva, serum, and whole blood. [Figure not available: see fulltext.].