Effects and mechanism of metal ions on enzymatic hydrolysis of wheat straw after pretreatment

Abstract

The effects and mechanism on the enzymatic hydrolysis of lignocellulose by metal ions were investigated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Fluorescence Spectra, and enzymatic kinetics and activity analyses of the enzymatic hydrolysis system. Fe3+ exerted the best enzymatic hydrolysis effect on pretreated wheat straw, and the highest reducing sugar conversion was 44.5%. SEM and FTIR indicated that alkali and ultrasonic pretreatments partially removed the lignin and hemicellulose from lignocellulose. DLS and fluorescence spectra results revealed that the electrostatic interaction between Fe3+ and the carboxyl group in the enzyme enlarged the enzyme's steric structure. The data on enzymatic kinetics displayed that Fe3+ increased the maximum rate of reaction by 33.9% and the Michaelis-Menten by 5.72 g/L. The enzyme activity increase rate rose initially and then diminished, and the maximum increase rate was 36.1%.