A wide variety of noncoding RNAs are thought to play fundamental and regulatory functions in the mammalian cell nucleus, although their primary functions have yet to be elucidated. Here, we describe a method for the rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells using chemically modified antisense oligonucleotides, which may facilitate the functional assessment of nuclear ncRNAs.
CITATION STYLE
Hirose, T., & Mannen, T. (2015). Rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells with antisense oligonucleotides. Methods in Molecular Biology, 1206, 149–156. https://doi.org/10.1007/978-1-4939-1369-5_13
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