THE EFFECT OF Δ1‐TETRAHYDROCANNABINOL ON THE UPTAKE OF [3H]‐(—)‐NORADRENALINE BY THE ISOLATED PERFUSED HEART OF THE RAT

Abstract

Forty‐eight isolated rat hearts were perfused with Krebs solution and with [3H]‐(–)‐noradrenaline ([3H]‐NA) containing 4.5 nCi/ml in 50 ng/ml, as described by Iversen (1963). To groups of six, cocaine was added to [3H]‐NA in concentrations of 400 Nm, 2 and 10 μm. To the rest Tween 80, 25 μg/ml was added to [3H]‐NA and in three groups Δ1‐THC additionally in concentrations of 560 Nm and 2.8 or 14 μm. Cocaine caused a linear concentration‐related inhibition of uptake; Tween caused a 38.7% inhibition; THC caused additionally a linear concentration‐related inhibition. 1974 British Pharmacological Society