Context - Intestinal inflammation can induce a local reduction in oxygen levels that triggers an adaptive response centered on the expression of hypoxia-inducible factors (HIFs). Nitric oxide, a well-described inflammatory mediator, may interfere with hypoxia signaling. Objectives - We aimed to evaluate the role of nitric oxide in hypoxia signaling during colonic inflammation. Method - Colitis was induced by single (acute) or repeated (reactivated colitis) trinitrobenzenosulfonic acid administration in rats. In addition, one group of rats with reactivated colitis was also treated with Nw-Nitro-L-arginine methyl ester hydrochloride to block nitric oxide synthase. Colitis was assessed by macroscopic score and myeloperoxidase activity in the colon samples. Hypoxia was determined using the oxygen-dependent probe, pimonidazole. The expression of HIF-1a and HIF-induced factors (vascular endothelial growth factor - VEGF and apelin) was assessed using Western blotting. Results - The single or repeated administration of trinitrobenzenosulfonic acid to rats induced colitis which was characterized by a high macroscopic score and myeloperoxidase activity. Hypoxia was observed with both protocols. During acute colitis, HIF-1a expression was not increased, but VEGF and apelin were increased. HIF-1a expression was inhibited during reactivated colitis, and VEGF and apelin were not increased. Nw-Nitro-L-arginine methyl ester hydrochloride blockade during reactivated colitis restored HIF-1a, VEGF and apelin expression. Conclusions - Nitric oxide could interfere with hypoxia signaling during reactivated colitis inflammation modifying the expression of proteins regulated by HIF-1a.
CITATION STYLE
Gambero, A., Moscato, C. H., Tomé, R. B. G., Pedrazzoli, J., & Ribeiro, M. L. (2014). Nitric oxide interferes with hypoxia signaling during colonic inflammation. Arquivos de Gastroenterologia, 51(4), 302–308. https://doi.org/10.1590/s0004-28032014000400007
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