Anti-diabetic and hepato-renal protective effects of ziyuglycoside II methyl ester in type 2 diabetic mice

35Citations
Citations of this article
22Readers
Mendeley users who have this article in their library.

Abstract

Type 2 diabetes is a metabolic disorder caused by abnormal carbohydrate metabolism, and closely associated with abnormal lipid metabolism and hepato-renal dysfunction. This study investigated the anti-diabetic and hepato-renal protective properties of ziyuglycoside I (ZG01) derivative on type 2 diabetes. ZG01 was isolated from roots of Sanguisorba officinalis and chemically modified by deglycosylation and esterification to obtained ziyuglycoside II methyl ester (ZG02-ME). Here, we showed that ZG02-ME has stronger anti-diabetic activity than the original compound (ZG01) through decreasing blood glucose, glycated hemoglobin (HbA1c), and insulin levels in a mouse model of type 2 diabetes (db/db mice). We further found that ZG02-ME treatment effectively ameliorated serum insulin, leptin and C-peptide levels, which are key metabolic hormones, in db/db mice. In addition, we showed that elevated basal blood lipid levels were decreased by ZG02-ME treatment in db/db mice. Furthermore, treatment of ZG02-ME significantly decreased serum AST, ALT, BUN, creatinine, and liver lipid peroxidation in db/db mice. These results demonstrated that compared to ZG01, chemically modified ZG02-ME possess improved anti-diabetic properties, and has hepato-renal protective activities in type 2 diabetes.

Figures

  • Figure 1. Structure of ziyuglycoside I and ziyuglycoside II methyl ester. (1) ziyuglycoside I (ZG01); (2) ziyuglycoside II methyl ester (ZG02-ME).
  • Table 1. Effect of treatment of ziyuglycosides on glucose, HbAlc, and insulin levels in diabetic-obese (db/db) mice.
  • Table 2. Effect of ZG02-ME on blood glucose and HbAlc levels in diabetic-obese (db/db) mice.
  • Table 3. Effect of ZG02-ME on serum insulin, leptin, and C-peptide levels in diabetic-obese (db/db) mice.
  • Figure 2. The effects of ZG02-ME administration on blood lipid levels, body weight gain, and food consumption in diabetic-obese (db/db) mice. The db/m mice were intragastrically administrated daily with normal saline containing 0.01% DMSO, as the vehicle, for four weeks (normal control). The db/db mice were intragastrically administrated daily with vehicle (vehicle control) or ZG02-ME (1, 3, 5 mg/kg body weight), respectively, for four weeks. (A) The total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL-C) levels in blood serum were measured as described in the methods; (B) Body weight and food intake were continuously measured from initial to final administration. Data are shown as mean ± SD of (n = 8). # p < 0.05 vs. normal control, and * p < 0.05 vs. vehicle-treated control as determined by one-way Student’s t-test.
  • Figure 3. The effects of ZG02-ME treatment on AST, ALT, creatinine, BUN, and TBARS in diabetic-obese (db/db) mice. The db/m mice were intragastrically administrated daily with normal saline containing 0.01% DMSO, as the vehicle, for four weeks (normal control, NC). The db/db mice were intragastrically administrated daily with vehicle (vehicle control, VC) or ZG02-ME (1, 3, 5 mg/kg body weight), respectively, for four weeks. (A) Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels as well as (B) creatinine (CRE) and blood urea nitrogen (BUN) levels in blood serum were measured; (C) Thiobarbituric acid reactive substances (TBARS) levels in liver and kidney tissues were also analyzed as described in the methods. Data are shown as mean ± SD of (n = 8). # p < 0.05 vs. normal control, and * p < 0.05 vs. vehicle-treated control as determined by one-way Student’s t-test.

References Powered by Scopus

1807Citations
20163Readers

This article is free to access.

This article is free to access.

This article is free to access.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Son, D. J., Hwang, S. Y., Kim, M. H., Park, U. K., & Kim, B. S. (2015). Anti-diabetic and hepato-renal protective effects of ziyuglycoside II methyl ester in type 2 diabetic mice. Nutrients, 7(7), 5469–5483. https://doi.org/10.3390/nu7075232

Readers over time

‘15‘16‘17‘18‘19‘20‘21‘22‘23‘24‘2501234

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

67%

Lecturer / Post doc 2

22%

Researcher 1

11%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 6

50%

Medicine and Dentistry 3

25%

Pharmacology, Toxicology and Pharmaceut... 2

17%

Agricultural and Biological Sciences 1

8%

Save time finding and organizing research with Mendeley

Sign up for free
0