Effects of Qinglongyi polysaccharide on complex biochemisty function with the core of band 3 on erythrocyte

Abstract

Objective: To explore the effects of Qing long yi polysaccharide on biochemical functions of band 3 core complex in S180 mouse erythrocyte membrane. Methods: We use SDS-PAGE method and G-250 dye to study the effect of qing long yi polysaccharide on band 3 content of S180 mouse erythrocyte, use LSCM to measure the concentration of H+ in erythrocyte of S180 mice, use fluorescence spectrophotometry to determine membrane fluorescence polarization and microviscosity, use spectrophotometer to measure sialic acid content on erythrocyte membrane, ATPase test kits to determine the activities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase, use FCM to measure membrane potential. Results: It showed medium(50mg/kg) and high-dosage (100mg/kg) of Qing long yi polysaccharide increased band 3 content. The result showed medium- dosage (50mg/kg) of Qing long yi polysaccharide signally increased band 3 function on CI- transportation, decreased the concentration of H +, decreased the concentration of Ca2+, increased membrane fluidity, increased sialic acid content, enhanced the activities of Na +,K+-ATPase and Ca2+,Mg2+-ATPase, heightened membrane potential. Conclusion: Qing long yi polysaccharide can improve biochemical functions of band 3 core complex in S180 mouse erythrocyte membrane, Which enhance the immunity function of erythrocytes, it may be one of the most important antitumor mechanisms. © 2008 Springer-Verlag.