Four genes affecting seed traits in soybeans map to linkage group F

Abstract

To map members of a family of genes affecting seed traits, the genotype T311 and cultivars Raiden and Keburi were used to develop two F2 populations; Raiden x T311 and Keburi x T311. T311 is a shriveled seed mutant and progeny of the cross between breeding lines AP2 and P2180. Raiden contains a null allele in the Gy4 gene, and thus lacks the glycinin subunit A5A4B3. Keburi contains a null allele in the Cgy1 gene, and thus lacks the α' subunit of β-conglycinin. The objectives of this research were to genetically map the location of the genes conferring the shriveled seed phenotype of T311 and the a' subunit of β-conglycinin from Keburi and to confirm the genetic location of Gy4. Genetic analysis showed that the shriveled seed trait is inherited as a single recessive gene with maternal effect. Linkage analysis mapped the shriveled seed gene (Shr) and the Cgy1 gene to linkage group F. Other seed trait genes, Gy5 (glycinin subunit) and B1 (seed coat bloom), were previously mapped to this linkage group. The significance of the cluster of these seed genes was discussed. This research also confirmed the previous mapping of Gy4.