The embryonic stem cell test is an animal-free alternative test method for developmental toxicity. Mouse embryonic stem cells are cultured in a hanging drop method to form embryoid bodies. These embryoid bodies, when plated on tissue culture dishes, differentiate to form contracting myocardial cell foci within 10 days. Inhibition of cardiomyocyte differentiation by test compounds serves as the end point of the assay, as monitored by counting contracting muscle foci under the microscope. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Schulpen, S. H. W., & Piersma, A. H. (2013). The embryonic stem cell test. Methods in Molecular Biology, 947, 375–382. https://doi.org/10.1007/978-1-62703-131-8_27
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