The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential.
CITATION STYLE
Nicholls, D. G. (2018). Fluorescence measurement of mitochondrial membrane potential changes in cultured cells. In Methods in Molecular Biology (Vol. 1782, pp. 121–135). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7831-1_7
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