Reconstitution of Infectivity and Transcriptase Activity of Homologous and Heterologous Viruses: Vesicular Stomatitis (Indiana Serotype), Chandipura, Vesicular Stomatitis (New Jersey Serotype), and Cocal Viruses

Abstract

RNA transcriptase activities have been reconstituted from fractionated components of four rhabdoviruses: vesicular stomatitis virus (VSV) (Indiana serotype), Cocal, VSV (New Jersey), and Chandipura viruses. Heterologous reconstitution of transcription has been observed between template and enzyme fractions of VSV Indiana and Cocal viruses but not to any significant extent between components of the other two viruses with VSV Indiana template or enzyme. Homologous reconstitution of infectivity has been obtained for each virus from their respective parts as well as heterologous reconstitution between Cocal and VSV Indiana components.